Entering edit mode
6.5 years ago
manuelmendoza
▴
50
Hello everyone!
I am a noob in bioinformatics. Now I am trying to run "new Tuxedo" protocol using HISAT. But I have the following problem:
Error: Encountered internal HISAT2 exception (#1) Command: /opt/cesga/hisat2/2.0.5/gcc/5.3.0/bin/hisat2-align-s --wrapper basic-0
-p 8 --dat -x GRCh38/grch38/genome -S Hsa002F_L.sam -U samples/original/females/lti/Hsa002F_L.fastq (ERR): hisat2-align exited with value 1
We have several transcriptomes sequenced using IonTorrent (single-end) from different biological conditions. I have tried to run HISAT with the following command:
hisat2 -p 8 --dat -x grch38/genome -U $SAMPLES/Hsa002F_L.fastq -S Hsa002F_L.sam
I have downloaded the index from HISAT webpage.
Thanks in advance.
