Question: single cell RNA-seq 10X genomics
0
gravatar for rob.costa1234
3.1 years ago by
rob.costa1234250
United States
rob.costa1234250 wrote:

I am getting some single cell RNA-seq data for different time points. Is there a standard work flow reported for such analysis? I know there are various packages for downstream analysis of scRNA like moncol but do we process 1X data in regular RNA seq or there are special preprocessing steps involved. Thanks

rna-seq • 1.6k views
ADD COMMENTlink modified 3.1 years ago • written 3.1 years ago by rob.costa1234250
1

You should use CellRanger if you have access to raw data.

ADD REPLYlink written 3.1 years ago by GenoMax92k

This paper is a good start to understand differences between single cell rna seq and bulk rna seq analysis.

Stegle, Oliver, Sarah A. Teichmann, and John C. Marioni. "Computational and analytical challenges in single-cell transcriptomics." Nature Reviews Genetics 16.3 (2015): 133-145.

ADD REPLYlink written 3.1 years ago by Javad120

just wondering, can we use single cell library prep(e.g., from 10x genomics) for bulkRNA seq?

ADD REPLYlink written 2.9 years ago by Micheal_Xu0
Please log in to add an answer.

Help
Access

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.
Powered by Biostar version 2.3.0
Traffic: 2118 users visited in the last hour