I am trying to find correlation between genes and fold change values of RNA seq data, using WGCNA. Module identification is my motivation. In this process I learnt that the minimum sample size for WGCNA is 15 and it is highly expected to get noise if it is less than 15. My final filtered data set is 1000 * 4 (1000 - genes and 4 - logFC value).
- Can I still use WGCNA, as my focus is only modules identification.
- If noise is must, what extent it would be biasing the results.
- Is it completely wrong to use WGCNA for this small sample size.
Please let me know.
Thanks in advance.