"Phylogenetic data analysis using R"
FINAL CALL - LAST FEW PLACES
Delivered by Dr. Emmanuel Paradis
This course will run from 28th January - 2nd February 2018 at SCENE field station, Loch Lomond, Glasgow.
The main objectives of the course are to teach the theoretical bases of phylogenetic analysis, and to give the ability to initiate a phylogenetic analysis starting from the files of molecular sequences until the interpretation of the results and the graphics. The introduction will cover a brief historical background and an overview of the different methods of phylogenetic inference. Different kinds of data will be considered, but with a special emphasis on DNA sequences. The software used will be based on R and several specialized packages (particularly ape and phangorn). Other software will be used (e.g., MUSCLE or Clustal) called from R. Overall, the course will cover almost all aspects of phylogenetic inference from reading/downloading the data to plotting the results. This course is intended for PhD and postgraduate students, researchers and engineers in evolutionary biology, systematics, population genetics, ecology, conservation.
Course content is as follows Day 1 • Refresher on R: data structures, data manipulation with the indexing system, scripts, using the help system. • Introduction to phylogenetic inference. • Basics on phylogenetic data (sequences, alignments, trees, networks, “splits”) and other data in R. • Reading / writing data from files or from internet. • Matching data. Manipulating labels. Subsetting data. • Main package: ape. Day 2 • Plotting and annotating trees. • Theory of sequence alignment. Comparing alignments. Graphical analyses of alignments. • Main packages: ape (with MUSCLE and Clustal). Day 3 • Theory and methods of phylogeny reconstruction. • Parsimony methods. • Evolutionary distances. • Distance-based methods: General principles and the main methods (NJ, BIONJ, FastME, MVR). • Methods for incomplete distances matrices (NJ, BIONJ, MVR*). Methods for combining several matrices (SDM). • Main packages: ape, phangorn. Day 4 • Theory of maximum likelihood estimation. • Application to phylogeny reconstruction. • Substitution models. • Tree space and topology estimation. • Main packages: ape, phangorn. Day 5 • Tree comparison, consensus methods. • Topological space and distances. • Bootstrap. • Bayesian methods.
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-- Oliver Hooker PhD. PR statistics
2017 publications -
Ecosystem size predicts eco-morphological variability in post-glacial diversification. Ecology and Evolution. In press.
The physiological costs of prey switching reinforce foraging specialization. Journal of animal ecology.
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