Question: gene expression and RNA-Seq
0
gravatar for qudrat
19 months ago by
qudrat70
NATIONAL INSTITUTE OF IMMUNOLOGY, INDIA
qudrat70 wrote:

Hello All, I want to know through RNA-Seq analysis whether a particular transcript is getting expressed in a tissue or not. Please suggest me how to proceed.

rna-seq gene • 553 views
ADD COMMENTlink modified 19 months ago by Devon Ryan90k • written 19 months ago by qudrat70
2
gravatar for Devon Ryan
19 months ago by
Devon Ryan90k
Freiburg, Germany
Devon Ryan90k wrote:

Have a look at the zFPKM paper. Conveniently, there's now even a bioconductor package available that implements the algorithm.

ADD COMMENTlink written 19 months ago by Devon Ryan90k

I understand that theses premises are only valid for human transcriptomes..

ADD REPLYlink written 19 months ago by Antonio R. Franco4.0k

I've not seen indication of that. It's a generic method that should be roughly applicable generally (keeping in mind that all methods are going to be a bit hand-wavy).

I should add that I used this once or twice for mouse data and seemed to get reasonable results.

ADD REPLYlink modified 19 months ago • written 19 months ago by Devon Ryan90k
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