microarray data analysis
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3.4 years ago
au.rinki.bio ▴ 10

hello, i am working on microarray data set and trying to find out differentially expressed genes. i have normalize microarray data using rma algorithm. i want to calculate fold change for selection of differentially expressed genes. rma algorithm give log2 transformed value, i am not confirm. then how i can calculate fold change?

microaaray • 1.1k views
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FYI, Intensity values are log transformed in last step of RMA i.e summarization.

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3.4 years ago
venu 6.8k

Most normalization algorithms (all ?) give log2 expression value 0-16. You can use these values to find differential genes implementing methods like limma. You might be interested to check following links

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