I used BBmerge to get a histogram of insert sizes in my sample. I have paired reads (2 X150) in the fastq files. I found that 45% of my reads in that sample have insert size less than 150. Average insert size is 158. Is it okay that 45 % of the reads have lower insert size than a single read.
Is a problem with library preparation ? Is there any biological or technological reason to prevent having longer insert size for Human. Like can we argue with company to get better longer insert sizes in the next samples?