Dear Biostars, Hi (not English. So, be ready for some language flaws)
I have two sequences (from de novo RNA-seq assembly), after blastN (and also blastX), they show similar results and annotations (vasotocin related),
but, when I use NCBI online "Align two or more sequences " ; the answer is: "No significant similarity found".
Why is that? My ssumption is this that as they show the same annotation and same protein products, there should be some similarity (I even hoped for exact 100% match!). Am I wrong?
NOTE: my 2 SEQs:
>seq1 TCTGGGGAGCCCACGTAGCAGCCCATCCCTTCCCCACAGCAGATACTGGGGCCAAAGCAG AGGCCCCGGTTTCCGGGGCCACATGACATGCACGGTCTTTGCAGATCAGGAAAAGAGCGC TTTCCGCCTCGCGGACAGTTCTGGATGTAGCATGCAGATGAGAGCGCGAGGAGCCCCAGG ACGCACAGAAGTGGAATAGTAGAATCTGGCATCTTGTTCCGTCTAAAGTGCTTCGTCCAA TTTGTACTGTAGGCTACGGCTACAACTGAACTCCTTCAAATGGCTCGT >seq2 GTGGTTGGTTACTGAGGTCTCCCTCTGCTGGTGGCATGTAGGATCCGCAGCAGGTCTCCT GCCAAACCACCCATTAAGGCAGCGTTCTGTTCGCTGGGTGACTGACGTTTACTGTCCTCT AGGCAGTCTGGGTCTAGCACACAACTCTCTGAGTCACAGCAGACTCCGGATGCAGCACAG CTTCCCTCAGAGCCACACACTCTTCCTCCAGCCTCGCAGGGGGAGGGCAGGTAGTTCTCC