I have a set of proteins which have a lot of cysteines (more than 20 in each). I have made homology models for these proteins but need to predict the disulfide bonds/connectivity. I tried using DISULFIND but it is unable to predict connectivity for sequences with more than 10 cysteines.
Has anyone encountered this problem before? What is the most accurate way to predict disulfide bonds for proteins which do not have crystal structures?