Question: De novo sequencing?
gravatar for Abdul Rafay Khan
3.2 years ago by
Karachi, PK
Abdul Rafay Khan1.1k wrote:

Why do we required de novo sequencing if almost all the organisms are being sequenced? Why Don't we only focus on reference based sequencing?

de novo sequencing • 533 views
ADD COMMENTlink written 3.2 years ago by Abdul Rafay Khan1.1k

if almost all

Not at all

ADD REPLYlink written 3.2 years ago by Buffo1.8k

Sequencing is neither reference based or de novo. It's how you analyse the data afterwards.

Even for well characterized genomes such as humans we can discover new things using de novo assembly, such as complex structural variation.

ADD REPLYlink written 3.2 years ago by WouterDeCoster45k

The point is there is structural variation in genome between organism from the same species , so you can speak about De novo sequencing for every organism to my point of view ! If you check all genome reference organism have N , so genomes are partially sequenced because of the limits of assembly like low complexity region for example.

ADD REPLYlink written 3.2 years ago by Titus910
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