My illumina paired end reads are 150 bases long.
I ran bbmerge to check the insert size. Following is the link for the screen shot.
How can I have an avg insert size of 243 and a maximum of 291 when my reads themselves are 300 (2*150)? Does insert size here mean the inner distance (distance between R1 and R2)? I'm aware that there's ambiguity in the definition of insert size.
So, shall I consider the avg insert size to be 243 + 150 + 150 = 540 ??