setting CPU and max memory for running trinity

Question

[rahmati.razieh83]

Hi everyone

I run Trinity with this command

cd $HOME module load bowtie2 SAMTools-1.4.1 Trinity-2.4.0 Trinity --seqType fq --left all.left.trimmed2.fq --right all.right.trimmed2.fq --CPU 40 --max_memory 40G --bflyCPU 30 --monitoring --bflyCalculateCPU > $HOME/trinity.log 2>&1

but after passing 25 hours it was crashed and I got an error in Log file.

[rahmati.razieh83]

according to my running command and the error I received, what is the problem? Does it need to I change the settings in command?

[rahmati.razieh83]

This is the error I have received

[GenoMax]

Please edit the original post to add new information. You should not use Submit Answer for this purpose to keep threads logically organized.

Answer 1

[GenoMax]

Trinity requires 1G of RAM per 1M Illumina PE reads. How much data do you have? Unless you post the exact error we have no way of knowing what is going on.

[rahmati.razieh83]

I edited my post and attached the error file.