I am going to have PE reads for human RNAseq (around 70 millions of reads). How can I predict whether my computer have enough disc space and memory to run mapping reads to reference genome with the use of TopHat or any other RNAseq mapping algorithm?
I would like to decide whether I need to use cloud for this calculation or I can make it on my local computer.
I have 1T disc space and 64GB or RAM, 10 cores.
Thank you in advance,