We have paired end RNAseq reads which were trimmed 15bp and 10bp at the beginning and end of each respective read. My question is if this is appropriate considering it seems that each read will now have an overhang compared to it's paired read. Wouldn't this make the paired reads mismatching simply because of the shift in read coverage on the respective strands?
Question: Slight confusion about trimming paired end reads in RNAseq experiment
5 months ago by
mforde84 • 1.1k
mforde84 • 1.1k wrote:
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