Hi, I read previous posts about multiple conditions testing. However, my case is a little bit different. I have 3 different groups (plus their controls) with unequal number of technical replicates, so I have trouble to how to compare them.
First I want to compare each treated sample with its control, then normalize them to each other.
Then compare each treated group with each other. (Do I really need that intra-normalization to compare each treated group)
Samples are like that:
treated2.1 treated2.2 control2
treated3.1 treated3.2 control3
I think the design below is not correct:
set condition treated1 treated1 treated control1 control1 control treated2.1 treated2 treated treated3.1 treated3 treated treated3.2 treated3 treated control2 control2 control control3 control3 control treated3.2 treated3 treated
Can you help me to fix this. Thanks