Off topic:htseq-count error on galaxy
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Entering edit mode
3.3 years ago
costarc • 0

I modified my gff3 file to have a "gene_id" attribute but i keep having the error message: Fatal error: Unknown error occured Error occured when processing GFF file (line 4 of file /galaxy-repl/main/files/022/697/dataset_22697294.dat): Feature den1; Parent=rna0; Dbxref=Geneden:110546218; gene_id= XM_021633030.1; gbkey=mRNA; gene=Serpinf2; pr

My gff3 file has the following attributes to exons:

NW_018657744.1 Gnomon exon 6016 6139 . - . "ad=den1; Parent=rna0; Dbxref=Geneden:110546218; gene_id= XM_021633030.1; gbkey=mRNA; gene=Serpinf2; product=serpin family F member 2%2C transcript variant X2 ; Gend=XM_021633030.1" NW_018657744.1 Gnomon exon 4956 5022 . - . "ad=den2; Parent=rna0; Dbxref=Geneden:110546218; gene_id= XM_021633030.1; gbkey=mRNA; gene=Serpinf2; product=serpin family F member 2%2C transcript variant X2 ; Gend=XM_021633030.1" NW_018657744.1 Gnomon exon 4746 4787 . - . "ad=den3; Parent=rna0; Dbxref=Geneden:110546218; gene_id= XM_021633030.1; gbkey=mRNA; gene=Serpinf2; product=serpin family F member 2%2C transcript variant X2 ; Gend=XM_021633030.1" NW_018657744.1 Gnomon exon 4567 4629 . - . "ad=den4; Parent=rna0; Dbxref=Geneden:110546218; gene_id= XM_021633030.1; gbkey=mRNA; gene=Serpinf2; product=serpin family F member 2%2C transcript variant X2 ; Gend=XM_021633030.1" NW_018657744.1 Gnomon exon 4123 4324 . - . "ad=den5; Parent=rna0; Dbxref=Geneden:110546218; gene_id= XM_021633030.1; gbkey=mRNA; gene=Serpinf2; product=serpin family F member 2%2C transcript variant X2 ; Gend=XM_021633030.1" NW_018657744.1 Gnomon exon 3535 3678 . - . "ad=den6; Parent=rna0; Dbxref=Geneden:110546218; gene_id= XM_021633030.1; gbkey=mRNA; gene=Serpinf2; product=serpin family F member 2%2C transcript variant X2 ; Gend=XM_021633030.1" NW_018657744.1 Gnomon exon 3182 3385 . - . "ad=den7; Parent=rna0; Dbxref=Geneden:110546218; gene_id= XM_021633030.1; gbkey=mRNA; gene=Serpinf2; product=serpin family F member 2%2C transcript variant X2 ; Gend=XM_021633030.1" NW_018657744.1 Gnomon exon 2387 2638 . - . "ad=den8; Parent=rna0; Dbxref=Geneden:110546218; gene_id= XM_021633030.1; gbkey=mRNA; gene=Serpinf2; product=serpin family F member 2%2C transcript variant X2 ; Gend=XM_021633030.1"

What can I do, please?

Thanks

RNA-Seq alignment software error • 1.3k views
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