This is the 3rd thread concerning this issue and we have made steady progress. I sincerely appreciate the inputs of cpad0112 and h.mon for their efforts
Have: * RNA expression data for 12 time points, 1 replicate * List of genes of interest and their expression data http://m.uploadedit.com/bbtc/1512924884525.txt [Sample data, does not contain all 100 genes]
Want: Expression pattern clustering like this where x axis is time and y axis is relative expression (or something like it)
Have tried: * Timecourse (R) which apparently does not accept if there is only 1 replicate [C: Cannot get result from TIMECOURSE (R PROGRAM)]
Question: Do you know of ANOTHER program that can achieve a similar result?
Possible solutions: 1) Take polynomial trend line of each line (gene) of expression data, cluster them accordingly manually. Problem: do not know how to get polynomial equation of each line of data using R.