Question: more than 28 million methylation sites?
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gravatar for christacaggiano
2.9 years ago by
UCSF
christacaggiano50 wrote:

Hi,

I am currently doing a whole genome bisulfite sequencing experiment. After running Bismark methylation caller and calculating percent methylation for each unique CpG, I am coming up with more than 46 million sites. I know that there are only 28 million sites in the genome, so I am very confused about my result. I was wondering if anyone had run into a similar issue after using Bismark?

Thanks!

cpg bismark bs-seq methylation • 805 views
ADD COMMENTlink modified 2.9 years ago by igor11k • written 2.9 years ago by christacaggiano50
0
gravatar for igor
2.9 years ago by
igor11k
United States
igor11k wrote:

A single CpG is actually two Cs if you consider both strands independently. Bismark will report a separate call for each C. Thus, you should expect twice as many sites.

ADD COMMENTlink written 2.9 years ago by igor11k
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