DESeq2, function not found

Question

[onemore100iq]

when I use this line

ddsHTSeq <- DESeqDataSetFromHTSeqCount(sampleTable = sampleTable, directory = directory, design= ~ subtype)

I got this error:

could not find function DESeqDataSetFromHTSeqCount

please help

[Devon Ryan]

Did you library(DESeq2)?

[WouterDeCoster]

I added (code) markup to your post for increased readability. You can do this by selecting the text and clicking the 101010 button. When you compose or edit a post that button is in your toolbar, see image below:

Did you load the DESeq2 library?

[Matteo Schiavinato]

In case it's the first time you use R: you need to load the packages every time you run the environment (that is: library(packagename). In this case: library("DESeq2").