How to compute phred score from a Sanger raw trace ?
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4.4 years ago
sacha ★ 2.3k

Hi,

I wonder how to detect peaks from a sanger raw trace file and compute phred scores for each base called.
For instance here is a vector of 2 peaks :

0
0
0
86
256
484
713
871
901
784
553
279
52
0
10
222
524
826
1033
1085
971
735
455
210
50
0
0


I can easily detect peaks using slope . But I wonder How to assign a phred score for each peaks. Any idea ?

sanger phred • 1.6k views
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