Question: How to compute phred score from a Sanger raw trace ?
0
gravatar for sacha
3.2 years ago by
sacha2.1k
France
sacha2.1k wrote:

Hi,

I wonder how to detect peaks from a sanger raw trace file and compute phred scores for each base called.
For instance here is a vector of 2 peaks :

0
0
0
86
256
484
713
871
901
784
553
279
52
0
10
222
524
826
1033
1085
971
735
455
210
50
0
0

I can easily detect peaks using slope . But I wonder How to assign a phred score for each peaks. Any idea ?

phred sanger • 1.2k views
ADD COMMENTlink modified 3.1 years ago by Biostar ♦♦ 20 • written 3.2 years ago by sacha2.1k
1

See if this helps: http://www.insilicase.com/Web/PhredScores.aspx

ADD REPLYlink written 3.2 years ago by GenoMax96k
1

Also take a look here: https://www.genewiz.com/Public/Resources/Tools-for-Viewing-Sequencing-Data/

ADD REPLYlink written 3.2 years ago by Kevin Blighe71k
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