Question: Doing RNA folding online for 1200 nt long sequence
0
gravatar for caggtaagtat
6 months ago by
caggtaagtat230
caggtaagtat230 wrote:

Hi,

I have a 1200 nt long RNA sequence and I would like to calculate the free energy for its structure, without downloading anything if possible.

rna-folding rna • 319 views
ADD COMMENTlink modified 6 months ago by Eric Lim600 • written 6 months ago by caggtaagtat230
3
gravatar for jrj.healey
6 months ago by
jrj.healey4.6k
United Kingdom
jrj.healey4.6k wrote:

There are many, many online webservers for this. Just google RNA folding webservers.

First result:

http://rna.tbi.univie.ac.at/cgi-bin/RNAWebSuite/RNAfold.cgi

ADD COMMENTlink written 6 months ago by jrj.healey4.6k

"Current limits are 7,500 nt for partition function calculations and 10,000 nt for minimum free energy"

Yeap, your sequence is not long enough to exceed the limits, go for it

ADD REPLYlink written 6 months ago by biofalconch370

Thanky you very much, I think my search missed the word "webserver".

Could you maybe also recommend a programm/package, to do the calculation automatically in R?

ADD REPLYlink written 6 months ago by caggtaagtat230

I’m afraid I personally don’t know of one (I’m not a heavy user of R for bioinformatics). There may be something in the bioconductor suite, you could start by looking for a vignette on their site perhaps,

ADD REPLYlink written 6 months ago by jrj.healey4.6k
0
gravatar for Eric Lim
6 months ago by
Eric Lim600
Stoke Therapeutics, Inc
Eric Lim600 wrote:

In addition to RNAfold,

Sfold: http://sfold.wadsworth.org/cgi-bin/index.pl

Mfold: http://unafold.rna.albany.edu/?q=mfold/RNA-Folding-Form

ADD COMMENTlink modified 6 months ago • written 6 months ago by Eric Lim600
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