Hello, I am doing RNAseq analysis for the first time. I have two samples, control and treatment of a plant variety collected at 14 days interval. I obtained few data sets of differentially expressed genes which had similar gene ID and were also the same transcripts. They were same except that, they differed in FPKM values and had different regulation, like one is up-regulated (12-fold) and other is down-regulated (13-fold). I assume that minor variation errors could be possible but such fold variation along with up-regulation and down-regulation cannot be overlooked. I also don’t think that they could be different fragments of the same transcript as they show different regulation. Can anyone suggest the reason for such data? Or is this mere an error.
Question: RNA seq result interpretation
2.5 years ago by
reena_gd • 0
reena_gd • 0 wrote:
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