Question: NGS to find SNPs
gravatar for DK
14 months ago by
DK80 wrote:

Dear All,

I am working on NGS analysis, I do not have much experience in this. I would really appreciate response from experienced personnels to share the steps of how to do NGS analysis to find SNPs effectively, where one is provided with fastq files of the reads.

Thanks much, DK

snp • 436 views
ADD COMMENTlink modified 14 months ago by Kevin Blighe39k • written 14 months ago by DK80
gravatar for genomax
14 months ago by
United States
genomax64k wrote:

GATK Best practices linked below glosses over alignments, which are obviously required to follow the steps in the guide. You can use any of the many NGS aligners available (BBMap, BWA, Bowtie2, if you are doing genome alignments) (BBMap, STAR, HISAT2 if you need a splice-aware aligner). Pick an aligner and then stick with it, once you understand its options (there are many for most and they are all program specific). You would also need access to a reference genome and will have to create search indexes (that are again aligner specific).

ADD COMMENTlink modified 14 months ago • written 14 months ago by genomax64k
gravatar for WouterDeCoster
14 months ago by
WouterDeCoster37k wrote:

Have a look at the GATK best practices.

ADD COMMENTlink written 14 months ago by WouterDeCoster37k
Please log in to add an answer.


Use of this site constitutes acceptance of our User Agreement and Privacy Policy.
Powered by Biostar version 2.3.0
Traffic: 900 users visited in the last hour