**440**wrote:

I am trying to automatically label some of the data points on a manhattan plot. For those who do not know what a manhattan plot is, it really does not matter. Rewriting the old style R code in ggplot2 seems to be a bigger challenging for me. Since the only feature I want to add to the script is to be able to automatically label some data points nicely (using ggrepel), so I think maybe I could just overlay the labels on the graph plotted with the old code.

My current try is as follows:

```
library(ggplot2);
library(ggrepel);
d=read.table("a.txt",header=T,fill=T, sep=" ");
dmht='';
dmht<-data.frame(chrom=d[,9], txStart = d[,11], "-log10(PValue)" = -log10(d[,5]))
# sort it
o<-order(dmht[,1],dmht[,2]);
dmht<-data.frame(dmht[o,]);
names(dmht)<-c("chrom", "txStart", "-log10(PValue)");
attach(dmht);
chrs<-c('chr1','chr2','chr3','chr4','chr5','chr6','chr7','chr8','chr9','chr10','chr11','chr12','chr13','chr14','chr15','chr16','chr17','chr18','chr19','chrX','chrY');
chrLabels = c('1', '2', '3', '4', '5', '6', '7', '8', '9', '10', '11', '12', '13', '14', '15', '16', '17', '18', '19', 'X', 'Y');
chrLen<-rep(0,length(chrs));
chrMin<-rep(0,length(chrs));
chrMax<-rep(0,length(chrs));
totalLen = 0;
for(i in 1:length(chrs)) {
dchr<-subset(dmht, chrom == chrs[i]);
chrMin[i]<-min(as.numeric(dchr[,2]),na.rm=T);
chrMax[i]<-max(as.numeric(dchr[,2]),na.rm=T);
chrLen[i]<-chrMax[i] - chrMin[i] + 1;
totalLen = totalLen + chrMax[i];
}
ds=read.table("selected2label.txt",header=T,fill=T,sep=" ");
dsel='';
dsel<-data.frame(ds[,9], ds[,11], -log10(ds[,5]) , ds[,1])
ymin = min(as.numeric(dmht[,3], dsel[,3]), na.rm=T);
ymax = max(as.numeric(dmht[,3], dsel[,3]), na.rm=T);
# chr start positions
chrStart<-rep(0,length(chrs));
for(i in 1:length(chrs)) {
if(i == 1) {
chrStart[i] = 1;
}
else {
chrStart[i] = chrStart[i-1] + chrMax[i-1] + 1;
}
}
#dmht=subset(dmht,dmht[,1] < 24); # remove mitochondra snps
colors <- rep(c("blue", "green", "cyan"),9);
png("result/test/mhtTest.png" , width=1600);
par(las=3, lab=c(length(chrLabels),5,7))
# draw the dots
dchr<-subset(dmht, chrom == chrs[1]);
plot(as.numeric(dchr[,2])+chrStart[1], as.numeric(dchr[,3]), col=colors[1], ylim=c(ymin,ymax),xlim=c(chrMin[1],totalLen),axes=F,ylab="-log10(PValue)", xlab="Chromosome", main="");
for(i in 2:length(chrs)) {
dchr<-subset(dmht, chrom == chrs[i]);
points(as.numeric(dchr[,2])+chrStart[i], as.numeric(dchr[,3]), col=colors[i]);
}
axis(side=1,labels=chrLabels,at=chrStart);
axis(side=2);
# draw the quantiles
quants<-quantile(as.numeric(dmht[,3]), p=c(),na.rm=T);
for(q in quants) {
abline(h=q);
}
# draw the abs values
abss<-c();
for(a in abss) {
abline(h=a);
}
# sort it
#os<-order(dsel[,1],dsel[,2]);
#dsel<-data.frame(dsel[os,]);
#dsel
#dsel<-data.frame(dsel);
#dsel
colnames(dsel)<-c("chrom", "txStart", "-log10(PValue)" , 'GENE_ID');
detach(dmht);
attach(dsel);
# highlight the selected dots
for(i in 1:length(chrs)) {
dchr<-subset(dsel, chrom == chrs[i] | paste("chr", chrom, sep="") == chrs[i]);
if(length(dchr[,2]) > 0) {
print(dchr)
# this is the new code
geom_label_repel(data = dchr, aes(label = GENE_ID, x=as.numeric(txStart)+chrStart[i], y = as.numeric(dchr[,3])), size = 5, box.padding = unit(0.35, "lines"), point.padding = unit(0.5, "lines"))
# replacing the line in the old script:
# text(as.numeric(dchr[,2])+chrStart[i], as.numeric(dchr[,3]), dchr[,4])
}
}
dev.off();
```

The only line I replaced (besides adding "libary(ggplot2)" & "library(ggrepel)") was:

```
# this is the new code
geom_label_repel(data = dchr, aes(label = GENE_ID, x=as.numeric(txStart)+chrStart[i], y = as.numeric(dchr[,3])), size = 5, box.padding = unit(0.35, "lines"), point.padding = unit(0.5, "lines"))
# replacing the line in the old script:
# text(as.numeric(dchr[,2])+chrStart[i], as.numeric(dchr[,3]), dchr[,4])
```

The problem is that the labelling does not show up at all.

Your help would be highly appreciated.