Question: DADA2 vs DEBLUR
0
gravatar for sara.dandreano
15 months ago by
sara.dandreano0 wrote:

Hi all!

I'm a PhD student working on Microbiome and Mycobiome in skin dog! I'm learning how to use QIIME2 for sequences analysis.

I saw that in QIIME2 you can use or DADA2 or Deblur for the analysis, and my question is: what do you think about them?? which one is better?

Thank you very much, any help will be very appreciated!

Best regards

Sara

sequencing next-gen sequence • 1.8k views
ADD COMMENTlink modified 15 months ago by arsilan32470 • written 15 months ago by sara.dandreano0

Thank you for your reply!

I obtained my sequencing data from IonTorrent and I'm sequencing the 16S gene. As I'm not an expert of DADA2 and Deblur, I asked opinions about them to understand if both were fine for these analysis!!

ADD REPLYlink written 15 months ago by sara.dandreano0

Americans are really preferring DADA2 for no reasons. Last month I attended this course QIIME2 with Antonio and Tomasz (who developed qiime) and they were all saying its your choice to choose. In any case it is the interpretation at the end. All the test sessions were also based on DADA2 and so I also used it for my dataset. The same recommendation I would give you as well!

best

ADD REPLYlink modified 15 months ago • written 15 months ago by arsilan32470

arsilan324 and sara.dandreano : Please use ADD COMMENT/ADD REPLY when responding to existing posts to keep threads logically organized.

Submit Answer should only be used for new answers to original question.

ADD REPLYlink modified 15 months ago • written 15 months ago by genomax65k

arsilan324 Really thank you for your reply!!!!!

ADD REPLYlink modified 15 months ago • written 15 months ago by sara.dandreano0

You are welcome! If you have any questions regarding workflow, you can reach me ..

ADD REPLYlink modified 15 months ago by genomax65k • written 15 months ago by arsilan32470

Please keep all discussion on the forum so it benefits everyone.

ADD REPLYlink modified 15 months ago • written 15 months ago by genomax65k

Ok so Deblur authors are not recommending Deblur in their workshops?

ADD REPLYlink written 10 months ago by jnesme0
2
gravatar for arsilan324
15 months ago by
arsilan32470
arsilan32470 wrote:

This is really personal choice and also based on your dataset. If your sequencing data is Illumina MiSeq/HiSeq I would suggesting using DADA2

ADD COMMENTlink written 15 months ago by arsilan32470
Please log in to add an answer.

Help
Access

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.
Powered by Biostar version 2.3.0
Traffic: 1229 users visited in the last hour