Entering edit mode
6.3 years ago
fiona.newberry
▴
80
Hi,
I am using SOAPaligner and soap2sam.pl to convert the output file to a sam file.
I then try to convert the supposed sam file to a bam file and get this error messages
[E::sam_parse1] missing SAM header
[W::sam_read1] parse error at line 1
[main_samview] truncated file.
This is what the soap alignment file looks like:
simulated.A.A.1.2618103/1 TGGAACAACTATACCAAACCAATTCAAAACGAAGAACTAACATCTTTAATTAGAGGAGCACAAACAGCAATGGATCAAACCGCAGAAGAAGAAATGGACT IIIIHHHIIGIHIGHIIIHFEHIGGFIIIIIIEEICIIIICIIGIIIIAIAHIBGE@DFIEI>EI;:E<IICIIIIIIIIIIII?D?F>IICIDBFF:II 1 a 100 + gi|9627186|ref|NC_001539.1| 401 2 A->82C9 T->29C9 100M 29T52A17
And the what the sam file looks like after soap2sam:
simulated.A.A.1.2618103 99 gi|9627186|ref|NC_001539.1| 401 30 100M = 568 267 TGGAACAACTATACCAAACCAATTCAAAACGAAGAACTAACATCTTTAATTAGAGGAGCACAAACAGCAATGGATCAAACCGCAGAAGAAGAAATGGACT IIIIHHHIIGIHIGHIIIHFEHIGGFIIIIIIEEICIIIICIIGIIIIAIAHIBGE@DFIEI>EI;:E<IICIIIIIIIIIIII?D?F>IICIDBFF:II NM:i:2 MD:Z:29T52A17
Obviously it is missing the normal lines you would see in sam file (@HD, @SQ, etc)
Does anyone know what is going on?