Errors about Tailseeker-3.1.7

Question

[11707072]

I used the Tailseeker-3.1.7 for the analysis of Tail-seq data. There is no error reported during runing. However it can not estimate the length of spike-ins rightly except spikein8 which maybe estimated by base-calling algorithm. BTW, I found that during the step of "process_signals" , it generate null pos_tile.sigdists. As a result, during the step of "calculate_optimal_parameters", it can only get NaN value for the cutoff. Then, this may contribute to the final result incorrect. Do you ever met this problem? Is there any problem in my settings?

My Settings:

include:
    - defaults.conf

run_id:         "ts001"

sources:
    - id:       a
      type:     HiSeq-RapidV2
      dir:      "150421_SN7001146_0233_BC6APYACXX"
      lane:     2

experimental_samples:
    Ctrl1: ATCACG
    Ctrl2: CGATGT
    dcKO1: TTAGGC
    dcKO2: TGACCA

reference_set:
    _exp: GRCm38

spikein_samples:
    spk8:  ACTTGA
    spk16: CTTGTA
    spk32: CGCTAA
    spk64: TCTGCT

spikein_lengths:
    spk8:  8
    spk16: 16
    spk32: 32
    spk64: 64

read_cycles:
    R5: [1, 100, 1]
    Ri: [101, 106, 2]
    R3: [110, 209, 3]

dupcheck_regions:
    _exp: [[21, 35], [110, 124]]

analysis_level: 1

performance:
    enable_gsnap: yes

third_party_basecaller:
    R5: AYB