Hi to all Good Afternoon I got data like this

```
1 2 3 4 5
A 0.1 0.2 0.3 0.5 0.6
B 0.7 0.8 0.9 1 1.1
C 1.2 1.3 1.4 1.5 1.6
D 1.7 1.8 1.9 2 2.1
E 2.2 2.3 2.4 2.5 2.6
```

and now I want to create lower triangle matrix like this

```
1 2 3 4 5 A B C D E
1 0
2 0.1 0
3 0.2 0.1 0
4 0.4 0.3 0.2 0
5 0.5 0.4 0.3 0.1 0
A 0.1 0.2 0.3 0.5 0.6 0
B 0.7 0.8 0.9 1 1.1 0.6 0
C 1.2 1.3 1.4 1.5 1.6 1.1 0.5 0
D 1.7 1.8 1.9 2 2.1 1.6 1 0.5 0
E 2.2 2.3 2.4 2.5 2.6 2.1 1.5 1 0.5 0
```

I just deducted distance between 2 from 1 from first table to get genetic distance between 1 and 2 (0.2 - 0.1=0.1) and like this I did for rest of the entries and I do not know doing like this is correct or not?, after doing calculation like that made lower triangle matrix. I tried like this in R x <- read.csv("AD2.csv", head = FALSE, sep = ",") b<-lower.tri(b, diag = FALSE) but I am getting only TRUE and FALSE as output not like distance matrix. can any one help to solve this problem and here is link to my example data https://www.dropbox.com/s/5yjo4w73pwlv51g/distance%20matrix.xlsx?dl=0 any help in this regard is highly appreciated Thanks in advance With Kind Regards