Dear all hi,
I was given a sequence of 1500 bps. I was asked to replace each amino acid with the rest 19 based on codon usage in human. To control what I have done, I tried to map them with bwa to my subject region and used blastn for the local alignment but for some regions i did not work.
my primer is consisted of 19 bp flanking regions as following.
19 bp + codon(19) + 19bp.
My aim is to visualise my primers correctly. Has anyone experience such a thing?
Thanks for the help,