Question: Stringtie/Cufflinks with yeast RNAseq
gravatar for grant.hovhannisyan
14 months ago by
grant.hovhannisyan1.5k wrote:

Hi Biostars,

I am working with yeast RNAseq data, and want to do guided transcriptome assembly using stringtie. Does anybody have experience with parameters of stringtie/stringtie-merge/gffcompare when working of yeast (almost no introns, short distance between genes)? For example, what are optimal values for minimum input transcript length to include in the merge, Maximum distance (range) for grouping transcript start sites, Maximum distance (range) allowed from free ends of terminal exons of reference transcripts when assessing exon accuracy?


ADD COMMENTlink modified 14 months ago • written 14 months ago by grant.hovhannisyan1.5k
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