I am new to use cnvkit and i read manual of cnvkit. I did not understand so many things. I want to used cnvkit to calculate copy number in my sequencing data with no normal sample. So i followed the steps that mentioned in manual. In my analysis, i have target file that include all the exon positions. From the target bed file, i got the antitarget file with antitarget command. Can someone explain me that what does mean of antitarget region. As i read, i got that region between target region is is so or not ?? and i also do not understand what is the role of access command ?? Can some one explain me with example ?? i will be grateful for your help.