I have a bam file from a cancer dataset and I want to convert it to FASTQ to check its quality. So I use this command samtools fastq #bamfile > cancer.fa. I intend to use this fastq file as an input to the FASTQC tool and get an idea about its quality. But these are paired end reads. So how can I get two read files (fastq) separately,(from a bam file) ? Is it ok to proceed with only one fastq file instead of two(for paired end reads) for quality analysis?
Question: (Closed) Bam to fastq conversion
2.6 years ago by
banerjeeshayantan • 170
banerjeeshayantan • 170 wrote:
ADD COMMENT • link •
Please log in to add an answer.
The thread is closed. No new answers may be added.
Powered by Biostar version 2.3.0
Traffic: 819 users visited in the last hour