How can i use BALIBASE to make comparison between (t-coffee,clustal,muscle)?step by step plz
How can i use BALIBASE to make comparison between (t-coffee,clustal,muscle)?step by step plz
HI 1-i used the data set
>seq0
FQTWEEFSRAAEKLYLADPMKVRVVLKYRHVDGNLCIKVTDDLVCLVYRTDQAQDVKKIEKF
>seq1
KYRTWEEFTRAAEKLYQADPMKVRVVLKYRHCDGNLCIKVTDDVVCLLYRTDQAQDVKKIEKFHSQLMRLME LKVTDNKECLKFKTDQAQEAKKMEKLNNIFFTLM
>seq2
EEYQTWEEFARAAEKLYLTDPMKVRVVLKYRHCDGNLCMKVTDDAVCLQYKTDQAQDVKKVEKLHGK
>seq3
MYQVWEEFSRAVEKLYLTDPMKVRVVLKYRHCDGNLCIKVTDNSVCLQYKTDQAQDVK
>seq4
EEFSRAVEKLYLTDPMKVRVVLKYRHCDGNLCIKVTDNSVVSYEMRLFGVQKDNFALEHSLL
>seq5
SWEEFAKAAEVLYLEDPMKCRMCTKYRHVDHKLVVKLTDNHTVLKYVTDMAQDVKKIEKLTTLLMR
>seq6
FTNWEEFAKAAERLHSANPEKCRFVTKYNHTKGELVLKLTDDVVCLQYSTNQLQDVKKLEKLSSTLLRSI
>seq7
SWEEFVERSVQLFRGDPNATRYVMKYRHCEGKLVLKVTDDRECLKFKTDQAQDAKKMEKLNNIFF
>seq8
SWDEFVDRSVQLFRADPESTRYVMKYRHCDGKLVLKVTDNKECLKFKTDQAQEAKKMEKLNNIFFTLM
>seq9
KNWEDFEIAAENMYMANPQNCRYTMKYVHSKGHILLKMSDNVKCVQYRAENMPDLKK
>seq10
FDSWDEFVSKSVELFRNHPDTTRYVVKYRHCEGKLVLKVTDNHECLKFKTDQAQDAKKMEK
2-used the online page of (clustal/t-coffee/muscle) to make MSA 3-get the output 4-then i used these outputs as an input in scorecones server i want to know the next step exactly how can i use BALIBASE to compare between (clustal/t-coffee/muscle) as i will draw a curve between the output of (clustal/t-coffee/muscle/balibase) the nearest one to balibase will be the best and where can i put the input (i want to work online)in balibase how can i align with BALIBASE
0.) Read How To Ask Good Questions On Technical And Scientific Forums
1.) read some background material (e.g. here, here, here, here)
2.) Download BALiBASE
3.) Install alignment programs
4.) Perform alignments with each alignment software
5.) Compute score for each alignment produced
6.) If getting errors: post new question, describing steps taken till tat point, commands used and error messages
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