Hi I have whole blood CpG from the 850K EPIC plateform. My goal is to try understand how deconvolusion works; that is would it be possible separate out the cpgs into different subtype and run a EWAS for each cell type. separately?
For example lets say I'm comparing group A to control using whole blood. I then run separate EWAS analysis on two of the major cell type, lets say T-Cell and PMBC. Is this possible? or is it more like I run an anlysis first to predict proportion and while running EWAS use that proportion as a covariate? Sorry this is new to me and I'm trying to understand it better.
Moreover, it would be great if someone can recommend a nice R package to do this would be great.