Question: detecting antisense transcripts
0
gravatar for vm.higareda
13 months ago by
vm.higareda20
vm.higareda20 wrote:

Hello

I am analyzing a single end strand specific (dUTP), transcriptome of D.melanogaster. I have already mapped and counted my sequences using:

hisat2 --rna-strandness R htseq-count -f bam -s reverse

Now, I would like to detect antisense transcripts How could do that?

Thanks

ADD COMMENTlink modified 13 months ago • written 13 months ago by vm.higareda20

SeqMonk has an antisense transcription pipeline you could try.

If you want to dissect the problem in more detail, you can try the approach from Antisense transcription - how to detect it?.

ADD REPLYlink written 13 months ago by h.mon24k
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