What genome assembler to choose for an unpaired FASTQ data derived from a Birch chloroplast?
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6.2 years ago
oxfordsoccer ▴ 10

What genome assembler to choose for an unpaired FASTQ data derived from a Birch chloroplast?

And other tools for pre- and post-processing also

File type Conventional base calls Encoding Sanger / Illumina 1.9 (actually Ion Torrent data) Total Sequences 376768 Sequences flagged as poor quality 0 Sequence length 5-376 %GC 36

chloroplast assembly fastq birch plant • 1.3k views
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You may want to try tadpole.sh from BBMap suite. It does well with small genomes. See guide here.

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Careful with this user.

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6.2 years ago
Asaf 10k

I'll go with SPAdes, try it with and without --meta

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