Question: Strange peaks in paired-end ChIP-Seq independent on Abs but not single-end or input
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gravatar for greulich.fr
21 months ago by
greulich.fr0 wrote:

Hello,

since we have switched to paired-end sequencing for ChIP-Seq, we observe strange peaks covering some of the expressed genes. See: https://ibb.co/mEf53x. These peaks differ, when performing ChIP-Seq against the same TF in different tissues, depending on gene expression, I guess. Published (single-end) data for the TF don't show these broad peaks at genes. Known enhancer peaks for this TF match the published data. So we gain this weared peaks additionally.

They appear consistently in ChIP-Seq Experiments with different Abs (same tissue) and also in KO samples, but not in the paired-end input (same tissue and sequencer) or single-end ChIP-Seq ; although performed in a different lab (different libary prep and different sequencer).

Is this an artefact of paired-end sequencing? Anyone observed similar peaks? Any suggestion is appreciated. Thanks Franzi

chip-seq • 374 views
ADD COMMENTlink written 21 months ago by greulich.fr0

The PE ChIP samples look similar to the SE samples, just smoothed out a bit, which makes sense if you're extending the coverage to account for fragments. My presumption is that your TF is interacting with the gene bodies or with the whole open chromatin region.

ADD REPLYlink written 21 months ago by Devon Ryan92k
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