In our trio data, we have identified germline denovo mutations in the child using a bioinformatics pipeline that we have 'assembled'. In general, some of those de novo mutations might have happened post-zygotically leading to embryonic mosaicism.
My question is, from the set of de novo mutations that we have, is there a way to know which ones are mosaic variants? maybe the latter are characterised by distinguishable allele frequencies?
would this be a good idea to try: run a somatic caller on my germline sample. The overlap between the detected somatic mutations and my previous set of de novo mutations could be considered as 'potential' mosaicism? I suggested this because mosaicism are considered as somatic mutations (i guess?) does this makes sense to try?