Question: Can we directly correlate FPKM/TPM to expression?
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gravatar for ag1805x
6 months ago by
ag1805x90
India
ag1805x90 wrote:

Can we directly correlate FPKM/TPM to expression? In other words, does high FPKM/TPM values mean high expression for a gene?

Or how do I find the highly expressed genes within a RNAseq sample?

sequencing rna-seq expession • 405 views
ADD COMMENTlink modified 6 months ago by noorpratap.singh110 • written 6 months ago by ag1805x90
3
gravatar for noorpratap.singh
6 months ago by
India
noorpratap.singh110 wrote:

FPKM/TPM are way of normalising the read counts. Link below explaining these: http://www.rna-seqblog.com/rpkm-fpkm-and-tpm-clearly-explained/

Now coming to other part, high FPKM/TPM values means high expression of a gene. But again this is relative to phenotype of interest and samples. If you have tumor and normal samples, an average FPKM higher value in tumors for certain genes means higher in tumors compared to normal would imply highly expressed genes in tumor.

ADD COMMENTlink modified 6 months ago • written 6 months ago by noorpratap.singh110
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