Question: Will pseudoaligners detect rearrangements based on known transcripts?
gravatar for b10hazard
22 months ago by
United States
b10hazard30 wrote:

I have a question about pseudo aligners. I’m using Kallisto and I was wondering if it would count novel rearrangements of known transcripts. For example, suppose I have the following gene consisting of three exons.


If I include this in the kallisto transcriptome and index it I understand that Kallisto will detect and count the intended transcript such as this…


My question is.... What if there were a transcript like this in which the second exon was missing….


Would Kallisto detect and include this in the gene counts?

What about if exon 2 and 3 were swapped, like this…


ADD COMMENTlink written 22 months ago by b10hazard30
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