sRNA data mapping to Rfam to find non coding RNA
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3.2 years ago

hi I am working on miRNA prediction. for that i want to remove non coding reads from my data by mapping it against Rfam database. so which data of Rfam database should be taken to map fasta sRNA sequence data ? I am confused Because .cm files have information about type of RNA i.e. sRNA/tRNA/snoRNA etc while fasta files of Rfam does not have this information. Can i take .cm files to make them database by Makeblastdb of NCBI blast tool. please give me suggestion. thanks a lot in advance.

rna-seq • 1.2k views
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