Question: What software to choose to do reference-assisted Birch Betula chloroplast genome assembly and subsequent annotation?
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gravatar for oxfordsoccer
13 months ago by
oxfordsoccer10
oxfordsoccer10 wrote:

What software to choose to do reference-assisted Birch Betula chloroplast genome assembly and subsequent annotation?

I have tried de novo assembly using BBMap, NextGene`s DeBrujin with various k-mers, Flotton and PE Flotton, then run Prokka and RAST on the resultant contigs. Did not apply scaffolding and gap closure tools. Prokka annotated some genes, RAST could not annotate anything. Does anybody have an experience of a tree chloroplast genome assembly and annotation? Thx. You forum is a good help.

plant tree assembly de novo • 651 views
ADD COMMENTlink written 13 months ago by oxfordsoccer10
1

IDBA-Hybrid is a good reference assisted genome assembly tool and if you haven't already tried it, RATT can be used for annotation transfer.

ADD REPLYlink modified 13 months ago • written 13 months ago by Sej Modha4.1k

I doubt Prokka and RAST will do a good job at annotating chloroplasts, you may try the tools listed here.

ADD REPLYlink written 13 months ago by h.mon24k
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