Functional annotation and quantifying genes

Question

[h.l.wong]

Hi all,

I have assembled some metagenomic contigs from some soil samples and have used prodigal to get the amino acid sequences (faa files).

I would like to functional annotate the contigs against kegg, dbcan, and pfam. What programs can I use to blast against these database? I can't use the web based database as my files are too large.

Afterwards I would like to quantify the annotated genes but I have no idea which programs to use.

Thank you for your time.

Cheers

Alan

[Mehmet]

Please have a look Blas2GO program.

https://www.blast2go.com

or David program:

https://david.ncifcrf.gov