Hi everyone! I have just finished a hybrid assembly on a plant genome using 40x PE Illumina data and 10x PacBio reads (10-20 kb). After hybrid assembly and polishing with DBG2OLC and blasr, I got 3645 backbones (is it the same as scaffolds?) with an N50 of 294 Kb. I was wondering how to further improve this assembly. Should I do further scaffolding or gap-filling? What tools do you suggest for that? Should I mask for repeats? Thanks a lot for your help!
Question: Should I do further scaffolding or gap-filling after hybrid assembly with DBG2OLC?
13 months ago by
pgaiero • 0
pgaiero • 0 wrote:
ADD COMMENT • link •
Please log in to add an answer.
Powered by Biostar version 2.3.0
Traffic: 1112 users visited in the last hour