Question: Err:510 in MISA tool
0
gravatar for mail2steff
14 months ago by
mail2steff50
Potsdam, Germay
mail2steff50 wrote:

Dear all,

I am trying to identify microsatellites in my sample using MISA tool. I executed and got the results. But when I opened the .statistics file I got error 510. I searched in google but couldnt get that.

Specifications
Err:510
Sequence source file: "SO_6823_RP.fasta"
Definement of microsatellites (unit size / minimum number of repeats):
(1/10) (2/6) (3/5) (4/5) (5/5) (6/5) 
Maximal number of bases interrupting 2 SSRs in a compound microsatellite:  100

RESULTS OF MICROSATELLITE SEARCH
Err:510

Total number of sequences examined:              4554886
Total size of examined sequences (bp):           7490335571
Total number of identified SSRs:                 1788202
Number of SSR containing sequences:              1141579
Number of sequences containing more than 1 SSR:  387133
Number of SSRs present in compound formation:    299506

why I am getting that err: 510?

microsatellite misa • 515 views
ADD COMMENTlink modified 14 months ago • written 14 months ago by mail2steff50

You don't provide enough information. What operating system do you use ? What command did you run ? Did you get any error message ?

ADD REPLYlink written 14 months ago by Jean-Karim Heriche18k

OS: Ubundu Command : perl misa.pl file.fasta I did not get any error message. But in the output file, file.statistics I got this error 510

ADD REPLYlink modified 14 months ago • written 14 months ago by mail2steff50

How did you open the file to look at its content ?

ADD REPLYlink written 14 months ago by Jean-Karim Heriche18k
0
gravatar for Jean-Karim Heriche
14 months ago by
EMBL Heidelberg, Germany
Jean-Karim Heriche18k wrote:

This looks like some other process has written to the file. MISA is a perl script and there's no way I can see for it to produce this.

EDIT: Another possibility is that the program used to open the file has an issue reading it.

ADD COMMENTlink modified 14 months ago • written 14 months ago by Jean-Karim Heriche18k
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