Hi Dear one,
I have 50 single cell RNA seq data of neuronal cells, I want to find the differential gene expression between the sub groups!
As for now I have calculated expression counts (in gene and isoform level) using RSEM ( I am planning to use EBSeq for differential gene expression), Can anyone guide me to do subgrouping (clustering) from this RSEM output? or any other best way?
thanks in advance