Question: Assembly of 454 raw data (in fastq)
gravatar for bird77
10 months ago by
bird7720 wrote:

I need to assemble raw reads from a project that already has been published. The metagenome was sequenced with 454, but the files are only present in fastq format.

Is there an assembler which is up-to-date which can assemble long 454 reads with this quality profile? Can UDBA or Megahit deal with that?

Or does anyone have different recommendations?

Thank you for your help.

assembly • 501 views
ADD COMMENTlink modified 9 months ago by colindaven840 • written 10 months ago by bird7720

I thought (though I may well be wrong) that Velvet or SOAP was originally developed for 454 sequencing.

ADD REPLYlink written 10 months ago by jrj.healey9.2k
gravatar for colindaven
9 months ago by
Hannover Medical School
colindaven840 wrote:

Newbler always worked pretty well with 454. Otherwise CLC genomics was also easy.

Can Spades deal with it for a more modern option ?

ADD COMMENTlink written 9 months ago by colindaven840

I don't think so. I use SPAdes fairly frequently and never noticed any information suggesting it does, but I am guessing a bit.

ADD REPLYlink written 9 months ago by jrj.healey9.2k
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