I have a network that I split into 8 clusters using a clustering algorithm. I further calculated the hub genes and the network parameters of these clusters.I further used DAVID to go a Gene Ontology analysis of these 8 clusters.
I found the orthogonal genes in Drosophila of the genes in each cluster and using GENEMANIA created 8 corresponding clusters for Drosophila. I found the hub genes in these 8 clusters and did a Gene Ontology analysis as well. My main aim was to compare and see if the genes were part of the same gene ontology functions in both Drosophila and the first species that I had.
I am however now stuck as to what more I can do with these networks. Most papers I read finish off their research with GO analysis. What more do you suggest that I could do with my clusters. Any ideas are welcome. I have all the clusters on Cytoscape, so I can use the vast resources available on cytoscape for my work.