Question: DNA replication protein on negative strand
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gravatar for utkarsh.sood
9 months ago by
utkarsh.sood30
India
utkarsh.sood30 wrote:

Hi, I have a genome in which the chromosomal replication protein is on the negative strand with the following coordinates

Start   Stop       Strand   Annotation
1440641 1438221    -        DNA gyrase subunit B (EC 5.99.1.3)
1441747 1440638    -        DNA recombination and repair protein RecF
1442860 1441757    -        DNA polymerase III beta subunit (EC 2.7.7.7)
1444427 1442889    -        Chromosomal replication initiator protein DnaA

Can the replication initiation protein be present in the complement strand because all the other genomes on the internet have all these genes on the positive strand?

Kindly suggest!

Thanks

oric circular genome assembly • 260 views
ADD COMMENTlink modified 9 months ago by Kevin Blighe33k • written 9 months ago by utkarsh.sood30

because all the other genomes on the internet have all these genes on the positive strand

https://xkcd.com/285/

enter image description here

ADD REPLYlink written 9 months ago by Pierre Lindenbaum115k

$ mysql --user=genome --host=genome-mysql.soe.ucsc.edu -A -D hg19 -e 'select distinct chrom,txStart,txEnd,strand from knownGene limit 3'

+-------+---------+-------+--------+
| chrom | txStart | txEnd | strand |
+-------+---------+-------+--------+
| chr1  |   11873 | 14409 | +      |
| chr1  |   14361 | 16765 | -      |
| chr1  |   14361 | 19759 | -      |
+-------+---------+-------+--------+
ADD REPLYlink written 9 months ago by Pierre Lindenbaum115k

What is this command for?

ADD REPLYlink written 9 months ago by utkarsh.sood30
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