DNA replication protein on negative strand
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3.8 years ago
utkarsh.sood ▴ 40

Hi, I have a genome in which the chromosomal replication protein is on the negative strand with the following coordinates

Start   Stop       Strand   Annotation
1440641 1438221    -        DNA gyrase subunit B (EC 5.99.1.3)
1441747 1440638    -        DNA recombination and repair protein RecF
1442860 1441757    -        DNA polymerase III beta subunit (EC 2.7.7.7)
1444427 1442889    -        Chromosomal replication initiator protein DnaA

Can the replication initiation protein be present in the complement strand because all the other genomes on the internet have all these genes on the positive strand?

Kindly suggest!

Thanks

Assembly Circular genome OriC • 667 views
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because all the other genomes on the internet have all these genes on the positive strand

https://xkcd.com/285/

enter image description here

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$ mysql --user=genome --host=genome-mysql.soe.ucsc.edu -A -D hg19 -e 'select distinct chrom,txStart,txEnd,strand from knownGene limit 3'

+-------+---------+-------+--------+
| chrom | txStart | txEnd | strand |
+-------+---------+-------+--------+
| chr1  |   11873 | 14409 | +      |
| chr1  |   14361 | 16765 | -      |
| chr1  |   14361 | 19759 | -      |
+-------+---------+-------+--------+
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What is this command for?

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